New PEO-IAA-Inspired Anti-Auxins: Synthesis, Biological Activity, and Possible Application in Hemp (Cannabis Sativa L.) Micropropagation

Abstract Auxins play an important role in plant physiology and are involved numerous aspects of development, such as cell division, elongation differentiation, fruit phototropic response. In addition, through their antagonistic interaction with cytokinins, auxins a key the regulation root growth apical dominance. Thanks to this capacity determine architecture, natural synthetic have been successfully employed obtain more economically advantageous plants. The crosstalk between cytokinins determines development thus is particular importance field micropropagation, where ratios these two phytohormones need be tightly controlled achieve proper rooting shoot generation. Previously reported anti-auxin PEO-IAA, which blocks auxin signalling binding TIR1 receptor inhibiting expression auxin-responsive genes, has used facilitate hemp micropropagation. Herein, we report set new PEO-IAA-inspired anti-auxins capable antagonizing responses vivo. compounds bind was confirmed vitro by SPR analysis. Using DESI-MSI analysis, evaluated uptake distribution at whole level. Finally, characterized effect on organogenesis explants, they showed able improve beneficial morphological traits, balanced all produced shoots enhanced bud proliferation.